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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: A Short Half-Life α IIb β 3 Antagonist ANTP266 Reduces Thrombus Formation
doi: 10.3390/ijms19082306
Figure Lengend Snippet: ANTP266 binding to integrin α IIb β 3 . ( A ) Chemical structure of naphthalene derivative ANTP266. ( B ) Effect of ANTP266 on the binding of fibrinogen to integrin α IIb β 3 . Purified human integrin α IIb β 3 with or without ANTP266 was added to wells coated with fibrinogen for 2 h at 37 °C, followed by adding a mouse anti-human integrin β 3 antibody. The binding of fibrinogen to α IIb β 3 was determined using anti-mouse IgG-conjugated alkaline phosphatase and disodium 4-nitrophenyl substrate at 405 nm. All experiments were performed in triplicate. ( C ) Docking mode of ANTP266 to integrin α IIb β 3 using CDOCKER in Discovery studio 3.0.
Article Snippet: The binding modes of ligands (ANTP266 and tirofiban) with α IIb β 3 were analyzed by
Techniques: Binding Assay, Purification